MS analysis and molecular characterization of Botrytis cinerea protease Prot-2. Use in bioactive peptides production.
Identifieur interne : 000190 ( Main/Exploration ); précédent : 000189; suivant : 000191MS analysis and molecular characterization of Botrytis cinerea protease Prot-2. Use in bioactive peptides production.
Auteurs : Ferid Abidi [Tunisie] ; Nayssene Aissaoui ; Jean-Charles Gaudin ; Jean-Marc Chobert ; Thomas Haertlé ; Mohamed Nejib MarzoukiSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Antibactériens (composition chimique), Antibactériens (pharmacologie), Antifongiques (composition chimique), Antifongiques (pharmacologie), Botrytis (enzymologie), Botrytis (génétique), Cadres ouverts de lecture (MeSH), Candida albicans (effets des médicaments et des substances chimiques), Chymotrypsine (composition chimique), Chymotrypsine (pharmacologie), Dosages enzymatiques (MeSH), Hydrolyse (MeSH), Metarhizium (enzymologie), Muscles (composition chimique), Peptide hydrolases (génétique), Peptide hydrolases (isolement et purification), Peptide hydrolases (pharmacologie), Peptides antimicrobiens cationiques (isolement et purification), Peptides antimicrobiens cationiques (pharmacologie), Perciformes (MeSH), Phyllachorales (enzymologie), Protéines fongiques (génétique), Protéines fongiques (isolement et purification), Protéines fongiques (pharmacologie), Staphylococcus aureus (effets des médicaments et des substances chimiques), Streptomyces (enzymologie), Séquence d'acides aminés (MeSH), Tests d'agents antimicrobiens par diffusion à partir de disques (MeSH).
- MESH :
- composition chimique : Antibactériens, Antifongiques, Chymotrypsine, Muscles.
- effets des médicaments et des substances chimiques : Candida albicans, Staphylococcus aureus.
- enzymologie : Botrytis, Metarhizium, Phyllachorales, Streptomyces.
- génétique : Botrytis, Peptide hydrolases, Protéines fongiques.
- isolement et purification : Peptide hydrolases, Peptides antimicrobiens cationiques, Protéines fongiques.
- pharmacologie : Antibactériens, Antifongiques, Chymotrypsine, Peptide hydrolases, Peptides antimicrobiens cationiques, Protéines fongiques.
- Animaux, Cadres ouverts de lecture, Dosages enzymatiques, Hydrolyse, Perciformes, Séquence d'acides aminés, Tests d'agents antimicrobiens par diffusion à partir de disques.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Anti-Bacterial Agents (chemistry), Anti-Bacterial Agents (pharmacology), Antifungal Agents (chemistry), Antifungal Agents (pharmacology), Antimicrobial Cationic Peptides (isolation & purification), Antimicrobial Cationic Peptides (pharmacology), Botrytis (enzymology), Botrytis (genetics), Candida albicans (drug effects), Chymotrypsin (chemistry), Chymotrypsin (pharmacology), Disk Diffusion Antimicrobial Tests (MeSH), Enzyme Assays (MeSH), Fungal Proteins (genetics), Fungal Proteins (isolation & purification), Fungal Proteins (pharmacology), Hydrolysis (MeSH), Metarhizium (enzymology), Muscles (chemistry), Open Reading Frames (MeSH), Peptide Hydrolases (genetics), Peptide Hydrolases (isolation & purification), Peptide Hydrolases (pharmacology), Perciformes (MeSH), Phyllachorales (enzymology), Staphylococcus aureus (drug effects), Streptomyces (enzymology).
- MESH :
- chemical , chemistry : Anti-Bacterial Agents, Antifungal Agents, Chymotrypsin.
- chemical , genetics : Fungal Proteins, Peptide Hydrolases.
- chemical , isolation & purification : Antimicrobial Cationic Peptides, Fungal Proteins, Peptide Hydrolases.
- chemical , pharmacology : Anti-Bacterial Agents, Antifungal Agents, Antimicrobial Cationic Peptides, Chymotrypsin, Fungal Proteins, Peptide Hydrolases.
- chemistry : Muscles.
- drug effects : Candida albicans, Staphylococcus aureus.
- enzymology : Botrytis, Metarhizium, Phyllachorales, Streptomyces.
- genetics : Botrytis.
- Amino Acid Sequence, Animals, Disk Diffusion Antimicrobial Tests, Enzyme Assays, Hydrolysis, Open Reading Frames, Perciformes.
Abstract
Prot-2 protease previously purified to homogeneity from Botrytis cinerea showed potentiality to be used in detergency and for production of bioactive peptides. To extend the characterization of Prot-2 protease, antifungal and antibacterial assays were performed in vitro using protein hydrolysates prepared from muscle of mackerel (Scomber scomborus) treated with this enzyme. The most active hydrolysate (degree of hydrolysis of 8 %) exhibited inhibition effect towards bacteria and phytopathogenic fungi, demonstrating that Prot-2 proteolysis generated bioactive peptides. Biochemical and molecular characterization of the purified Prot-2, by SDS-PAGE/Tryptic in gel-digestion and LC-MS/MS analysis, was investigated. The peptide amino acid sequence alignment search in database revealed a moderate homology between the determined amino acid sequence of Prot-2 protease and the known fungal trypsin/chymotrypsin in particular from Glomerella, Metarhizium and Streptomyces. From peptide sequence data obtained by mass spectrometry and sequences homologies, primers were defined and a cDNA fragment of 786 bp was amplified by RT-PCR. The cDNA nucleotide sequence analysis revealed an open reading frame coding for 262 amino acid residues. The deduced amino acid sequence of Prot-2 showed moderate identity with trypsin of Glomerella graminicola (74 %) and with chymotrypsin from Metarhizium anisopliae (71 %). Prot-2 exhibited a Ser protease homology and showed in addition the specific His motif of trypsin/chymotrypsin family.
DOI: 10.1007/s12010-013-0186-2
PubMed: 23494220
Affiliations:
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(pharmacology)</term><term>Hydrolysis (MeSH)</term><term>Metarhizium (enzymology)</term><term>Muscles (chemistry)</term><term>Open Reading Frames (MeSH)</term><term>Peptide Hydrolases (genetics)</term><term>Peptide Hydrolases (isolation & purification)</term><term>Peptide Hydrolases (pharmacology)</term><term>Perciformes (MeSH)</term><term>Phyllachorales (enzymology)</term><term>Staphylococcus aureus (drug effects)</term><term>Streptomyces (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Antibactériens (composition chimique)</term><term>Antibactériens (pharmacologie)</term><term>Antifongiques (composition chimique)</term><term>Antifongiques (pharmacologie)</term><term>Botrytis (enzymologie)</term><term>Botrytis (génétique)</term><term>Cadres ouverts de lecture (MeSH)</term><term>Candida albicans (effets des médicaments et des substances chimiques)</term><term>Chymotrypsine (composition chimique)</term><term>Chymotrypsine (pharmacologie)</term><term>Dosages enzymatiques (MeSH)</term><term>Hydrolyse (MeSH)</term><term>Metarhizium (enzymologie)</term><term>Muscles (composition chimique)</term><term>Peptide hydrolases (génétique)</term><term>Peptide hydrolases (isolement et purification)</term><term>Peptide hydrolases (pharmacologie)</term><term>Peptides antimicrobiens cationiques (isolement et purification)</term><term>Peptides antimicrobiens cationiques (pharmacologie)</term><term>Perciformes (MeSH)</term><term>Phyllachorales (enzymologie)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (isolement et purification)</term><term>Protéines fongiques (pharmacologie)</term><term>Staphylococcus aureus (effets des médicaments et des substances chimiques)</term><term>Streptomyces (enzymologie)</term><term>Séquence d'acides aminés (MeSH)</term><term>Tests d'agents antimicrobiens par diffusion à partir de disques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" 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fongiques</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Peptide hydrolases</term><term>Peptides antimicrobiens cationiques</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Antifongiques</term><term>Chymotrypsine</term><term>Peptide hydrolases</term><term>Peptides antimicrobiens cationiques</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Disk Diffusion Antimicrobial Tests</term><term>Enzyme Assays</term><term>Hydrolysis</term><term>Open Reading Frames</term><term>Perciformes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Dosages enzymatiques</term><term>Hydrolyse</term><term>Perciformes</term><term>Séquence d'acides aminés</term><term>Tests d'agents antimicrobiens par diffusion à partir de disques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Prot-2 protease previously purified to homogeneity from Botrytis cinerea showed potentiality to be used in detergency and for production of bioactive peptides. To extend the characterization of Prot-2 protease, antifungal and antibacterial assays were performed in vitro using protein hydrolysates prepared from muscle of mackerel (Scomber scomborus) treated with this enzyme. The most active hydrolysate (degree of hydrolysis of 8 %) exhibited inhibition effect towards bacteria and phytopathogenic fungi, demonstrating that Prot-2 proteolysis generated bioactive peptides. Biochemical and molecular characterization of the purified Prot-2, by SDS-PAGE/Tryptic in gel-digestion and LC-MS/MS analysis, was investigated. The peptide amino acid sequence alignment search in database revealed a moderate homology between the determined amino acid sequence of Prot-2 protease and the known fungal trypsin/chymotrypsin in particular from Glomerella, Metarhizium and Streptomyces. From peptide sequence data obtained by mass spectrometry and sequences homologies, primers were defined and a cDNA fragment of 786 bp was amplified by RT-PCR. The cDNA nucleotide sequence analysis revealed an open reading frame coding for 262 amino acid residues. The deduced amino acid sequence of Prot-2 showed moderate identity with trypsin of Glomerella graminicola (74 %) and with chymotrypsin from Metarhizium anisopliae (71 %). Prot-2 exhibited a Ser protease homology and showed in addition the specific His motif of trypsin/chymotrypsin family.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Aissaoui, Nayssene" sort="Aissaoui, Nayssene" uniqKey="Aissaoui N" first="Nayssene" last="Aissaoui">Nayssene Aissaoui</name><name sortKey="Chobert, Jean Marc" sort="Chobert, Jean Marc" uniqKey="Chobert J" first="Jean-Marc" last="Chobert">Jean-Marc Chobert</name><name sortKey="Gaudin, Jean Charles" sort="Gaudin, Jean Charles" uniqKey="Gaudin J" first="Jean-Charles" last="Gaudin">Jean-Charles Gaudin</name><name sortKey="Haertle, Thomas" sort="Haertle, Thomas" uniqKey="Haertle T" first="Thomas" last="Haertlé">Thomas Haertlé</name><name sortKey="Marzouki, Mohamed Nejib" sort="Marzouki, Mohamed Nejib" uniqKey="Marzouki M" first="Mohamed Nejib" last="Marzouki">Mohamed Nejib Marzouki</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Abidi, Ferid" sort="Abidi, Ferid" uniqKey="Abidi F" first="Ferid" last="Abidi">Ferid Abidi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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